Carpophores of ten Korean strains of Lentinus edodes (Berk.) Singer, an antitumor polysaccharide producing fungus, were extracted with 0.1N NaOH solution. The extracts were dialized for seven days in distilled water and lyophilized to produce crude polysaccharide powders. Thus obtained crude polysaccharide samples were assayed for sugar contents by calorimetric method with anthrone reagent. Among ten strains examined Lentinus edododes-DMC7 was found to be the richest strain in polysaccharide content of carpophores. By shake culture experiment for biomass production, L. cdodes-DMC7 was found to be the second most productive strain among seven strains examined.
Cultural characteristics of L. edodes-DMC7 were investigated by shake culture method. The best result was obtained when L. edodes-DMC7 was cultured in the medium containing glucose 8g, starch 80g, yeast extract 12g, KH₂PO₄, 0.87g, MgSO₄·7H₂O, 0.5g, CuCl₂ 0.3g, FeSO₄·7H₂O 10㎎ ZnSO₄·7H₂O 4㎎, CuSO₄·5H₂O 1㎎, MnCl₂·4H₂O 7㎎ per 11 at 28℃, 180 rpm, for 12 days. Thus thirty-three grams of dry mycelia was obtained per one liter of medium.
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